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LEDs are widely applied in highways and tunnels for their long life, low light attenuation, and being environment friendly in recent years. The influence of correlated color temperature (CCT) of LED on lighting safety has attracted people’s attention as the demand increased. In this paper, a calculation model of perception luminance of human eye considering mesopic vision and fog concentration was proposed. The influence of different CCTs on perception luminance of human eye under different levels of fog concentration and luminance was calculated and analyzed. The CCT of LED employed in the interior zone of a tunnel was selected based on the highest perception luminance in mesopic vision. Seven kinds of LEDs with different CCTs (3000–6500K) were applied in the experimental system with adjustable fog concentrations. The results showed that the main factors affecting visual perception are luminance and fog concentration. Higher luminance or lower fog concentration provides drivers with higher perception luminance. In contrast, although CCT has a less effect on perception luminance, LEDs with higher CCT (about 6500K) can provide higher perception luminance considering fog in mesopic vision. This paper provides technical support for tunnel lighting and guaranteeing traffic safety.