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The aim of this paper is to identify which factors have contributed to the success of Ruta N. Ruta N fostered the innovation ecosystem of Medellín, but until now it remains open how it happened from the inside perspective, which actors were involved in the process and which practices were made. Secondary data collection, interviews with directors directly involved and technical visits were made, and some important points emerged from the field that can shed light in order to apply those practices and configurations intentionally in other cities with similar contexts.