Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
oleh: Hendrik Deschout, Tomas Lukes, Azat Sharipov, Daniel Szlag, Lely Feletti, Wim Vandenberg, Peter Dedecker, Johan Hofkens, Marcel Leutenegger, Theo Lasser, Aleksandra Radenovic
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2016-12-01 |
Deskripsi
Live cell super-resolution imaging requires a high temporal resolution, which remains a challenge. Here the authors combine photo-activated localization microscopy (PALM) with super-resolution optical fluctuation imaging (SOFI) to achieve high spatiotemporal resolution and quantitative imaging of focal adhesion dynamics.