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Plectranthus amboinicus (Lour.) Spreng. has been used for the treatment of many diseases in traditional Indian systems of medicine. In the present study, Polyphenolic profiling was carried out in leaves of P. amboinicus using spectrophotometric and mass spectroscopic analyses. The total Polyphenolics of different extracts such as ethanol, acetone and n-butanol were estimated spectrophotometrically and major phenolic compounds of ethanol extract was characterized by HPLC coupled tandem mass spectroscopic analysis. Keywords: Plectranthus amboinicus, Phenolics, LC/MS