Find in Library
Search millions of books, articles, and more
Indexed Open Access Databases
Creation of mouse TNFR2-selective agonistic TNF mutants using a phage display technique
oleh: Daisuke Ando, Daisuke Ando, Daisuke Ando, Masaki Inoue, Masaki Inoue, Masaki Inoue, Haruhiko Kamada, Haruhiko Kamada, Haruhiko Kamada, Shintaro Taki, Shintaro Taki, Takeshi Furuya, Takeshi Furuya, Yasuhiro Abe, Kazuya Nagano, Kazuya Nagano, Kazuya Nagano, Yasuo Tsutsumi, Yasuo Tsutsumi, Yasuo Tsutsumi, Shin-ichi Tsunoda, Shin-ichi Tsunoda, Shin-ichi Tsunoda, Shin-ichi Tsunoda, Shin-ichi Tsunoda
Format: | Article |
---|---|
Diterbitkan: | Elsevier 2016-09-01 |
Deskripsi
Tumor necrosis factor-α (TNF), which is an immuno-modulatory cytokine, has been suggested to cause inflammatory responses as well as protection against tissue dysfunction by binding two types of TNF receptor (TNFR1/TNFR2). However, the physiological effects of TNFR2-specific activation remain unclear. We therefore aimed to generate a TNF mutant with full TNFR2-selective agonist activity as a functional analytical tool. In this study, we utilized a phage display technique to create mouse TNFR2 (mTNFR2)-selective TNF mutants that bind specifically to mTNFR2 and show full bioactivity compared with wild-type TNF. A new phage library displaying TNF mutants was created, in which nine amino acid residues at the predicted receptor-binding site were randomized. From this library, an agonistic TNF mutant exhibiting high binding selectivity and bioactivity to mTNFR2 was isolated. We propose that this TNF mutant would be a powerful tool with which to elucidate the functional roles of mTNFR2.•We generated a TNF mutant with full TNFR2-selective agonist activity.•This mutant was identified using a phage display technique.•This agonist exhibited high binding selectivity and bioactivity to mouse TNFR2.•This would be a powerful tool to elucidate the functional roles of mouse TNFR2.