Characterization of Protocatechuate 4,5-Dioxygenase from <i>Pseudarthrobacter phenanthrenivorans</i> Sphe3 and In Situ Reaction Monitoring in the NMR Tube

oleh: Epameinondas Tsagogiannis, Elpiniki Vandera, Alexandra Primikyri, Stamatia Asimakoula, Andreas G. Tzakos, Ioannis P. Gerothanassis, Anna-Irini Koukkou

Format: Article
Diterbitkan: MDPI AG 2021-09-01

Deskripsi

The current study aims at the functional and kinetic characterization of protocatechuate (PCA) 4,5-dioxygenase (PcaA) from <i>Pseudarthrobacter phenanthrenivorans</i> Sphe3. This is the first single subunit Type II dioxygenase characterized in Actinobacteria. RT-PCR analysis demonstrated that <i>pca</i>A and the adjacent putative genes implicated in the PCA <i>meta</i>-cleavage pathway comprise a single transcriptional unit. The recombinant PcaA is highly specific for PCA and exhibits Michaelis–Menten kinetics with K<sub>m</sub> and V<sub>max</sub> values of 21 ± 1.6 μM and 44.8 ± 4.0 U × mg<sup>−1</sup>, respectively, in pH 9.5 and at 20 °C. PcaA also converted gallate from a broad range of substrates tested. The enzymatic reaction products were identified and characterized, for the first time, through in situ biotransformation monitoring inside an NMR tube. The PCA reaction product demonstrated a keto-enol tautomerization, whereas the gallate reaction product was present only in the keto form. Moreover, the transcriptional levels of <i>pca</i>A and <i>pca</i>R (gene encoding a LysR-type regulator of the pathway) were also determined, showing an induction when cells were grown on PCA and phenanthrene. Studying key enzymes in biodegradation pathways is significant for bioremediation and for efficient biocatalysts development.