Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex
oleh: Arash Ghodousi, S Vatani, Davood Darban-Sarokhalil, Maryam Omrani, AA Fooladi, Ad Khosaravi, Mohammad Mehdi Feizabadi
| Format: | Article |
|---|---|
| Diterbitkan: | Tehran University of Medical Sciences 2012-03-01 |
Deskripsi
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.