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Introduction: Tuberculosis (TB), as the most common cause of death from infectious disease in the world, is estimated to rank 10th in view of the global burden of disease. Due to the increasing prevalence of this disease and reduce the incidence of smear-positive pulmonary TB in the province, this study was designed to investigate the TB epidemiology and its affected factors in Hamadan province. Methods: In this study, 842 patients with TB during 2007 to 2013 referred to the health centers of the province were studied. All information of patients, including age, gender, place of residence and patients’ condition, were collected. Data was analyzed by Chi-square test using SPSS22 statistical software. Results: The mean age of patients was 54.01±21.75. Of patients, 425 individuals (50.4%) were female. 2.1% of patients were infected with HIV, 579 (68.7%) with pulmonary TB and 412 (71.1%) were smear-positive cases. There was no significant relationship between gender and type of TB (P=0.81). Between place of residence (urban-rural) and the type of TB a significant relationship was found (P< 0.005). Age and type of TB, as well, showed statistically significant correlation (P<0.01). Conclusion: In this study, most patients with TB were in the age group of over 70 years old. Therefore, it is recommended to do more accurate screening in this age group to reduce the incidence risk of disease. Considering the high prevalence of TB and HIV at the same time, training and preventive Proceedings to control tuberculosis is recommended.