Measuring expression heterogeneity of single-cell cytoskeletal protein complexes
oleh: Julea Vlassakis, Louise L. Hansen, Ryo Higuchi-Sanabria, Yun Zhou, C. Kimberly Tsui, Andrew Dillin, Haiyan Huang, Amy E. Herr
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2021-08-01 |
Deskripsi
Existing methods for multimeric protein complex quantification in single cells suffer from limited selectivity and sensitivity. Here the authors report Single-cell protein Interaction Fractionation Through Electrophoresis and immunoassay Readout (SIFTER) and use this to probe the effects of cellular stress.