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This study was undertaken to assess the seroprevalence of Brucella canis at a kennel in Iran. Serum samples (n=21) were examined for the presence of B. canis antibodies with immunochromatography assay. Out of these, 57.2% were positive for B. canis antibodies. All sero positive dogs were more than 2 years old. Of these, majority (75%) was female dogs and 66.6% had the history of late abortion between 40 and 55 days of gestational period accompanied with brownish or gray-green vaginal discharge. The aborted puppies usually appeared partially autolysed. Scrotal dermatitis and orchitis were the only signs in male dogs. Prevention measures were also performed to control the infection in this kennel.