Find in Library
Search millions of books, articles, and more
Indexed Open Access Databases
Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching
oleh: Karol Alfaro, Paulina Bustos, Ciara O´Sullivan, Pablo Conejeros
Format: | Article |
---|---|
Diterbitkan: | University of Zagreb Faculty of Food Technology and Biotechnology 2015-01-01 |
Deskripsi
A simple method to detect saxitoxin (STX), one of the main components of the paralytic shellfish poison from red tide, has been developed. By using a next generation dye for double-stranded DNA we were able to differentiate fluorescence from STX-binding aptamers when exposed to different concentrations of STX, suggesting a change in aptamer folding upon target binding. The developed method is extremely rapid, only requiring small sample volumes, with quantitative results in the concentration range of 15 ng/mL to 3 μg/mL of STX, with a detection limit of 7.5 ng/mL.