Synthetic chimeric nucleases function for efficient genome editing
oleh: R. M. Liu, L. L. Liang, E. Freed, H. Chang, E. Oh, Z. Y. Liu, A. Garst, C. A. Eckert, R. T. Gill
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-12-01 |
Deskripsi
CRISPR-Cas systems have well characterized, modular structures. Here the authors use that architecture to design a Cas12a library of 560 synthetic chimeras, with altered PAM preferences and specificities.