Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
oleh: Kendall R. Sanson, Ruth E. Hanna, Mudra Hegde, Katherine F. Donovan, Christine Strand, Meagan E. Sullender, Emma W. Vaimberg, Amy Goodale, David E. Root, Federica Piccioni, John G. Doench
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2018-12-01 |
Deskripsi
Genome-wide libraries for CRISPR knockout, interference, and activation have allowed the systemic interrogation of gene function. Here, the authors evaluate the Brunello CRISPRko library and introduce Dolcetto and Calabrese for CRISPRi and CRISPRa, respectively.