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Abstract This work demonstrates the design and straightforward syntheses of several novel probe-based on rhodamine B and 2-mercaptoquinoline-3-carbaldehydes as a naked-eye colorimetric probe, indicating a sensitive and selective recognition towards nickel (II) with a limit of detection 0.30 μmol L−1 (0.02 mg L−1). Further, by employing the oxidation property of hypochlorite (OCl−), this novel probe parallelly has been deployed to detect hypochlorite in laboratory conditions with a limit of detection of 0.19 μmol mL−1 and in living cells. Regarded to negligible cell toxicity toward mammalian cells, this probe has the potential to determine these analytes in in-vivo investigation and foodstuff samples.