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The first complete mitochondrial genome of the wolf-eel (Anarrhichthys ocellatus) was determined and analyzed in this work. It had a circular mapping molecular with the length of 16,506 bp and contained 12S and 16S rRNAs, 22 tRNAs, 13 protein-coding genes, and a 851 bp D-loop in the typical arrangement of the vertebrate consensus. Phylogenetic analysis of the mitochondrial genome sequences of 47 representative species within the order Perciformes suggests that A. ocellatus is closely related to the species in the family Anarhichadidae. Anarrhichthys ocellatus mitogenome can contribute to our understanding of the phylogeny and evolution of this species.