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In this study, cellulose and cellulose nanofibers (CNF) were extracted and prepared from cassava peels (CPs). The method of the cellulose extraction was performed by alkali treatment followed by a bleaching process. The CNF were prepared by mechanical disruption procedure (homogenization and ultrasonication), and the results were compared with a common acid hydrolysis procedure. The resulting cellulose and CNF from both procedures were then analyzed using FTIR, SEM, TEM, XRD, and TGA. The results show that cellulose and CNF were successfully prepared from both procedures. The physical properties of the produced CNF were different; however, they hadsimilar chemical properties.