Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing
oleh: Thomas A. Hamilton, Gregory M. Pellegrino, Jasmine A. Therrien, Dalton T. Ham, Peter C. Bartlett, Bogumil J. Karas, Gregory B. Gloor, David R. Edgell
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2019-10-01 |
Deskripsi
CRISPR nucleases can be programmed to cleave sequences in specific bacteria to induce cell death. Here, Hamilton et al. present an optimized method for conjugative delivery of CRISPR nucleases, consisting of a single plasmid that encodes both the conjugative machinery and the nuclease.