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Photosynthetic efficiency is significantly affected by both qualitative and quantitative changes during light exposure. The properties of light have a profound effect on electron transport and energy absorption in photochemical reactions. In addition, fluctuations in light intensity and variations in the spectrum can lead to a decrease in photosystem II efficiency. These features necessitate the use of a simple and suitable tool called chlorophyll <i>a</i> fluorescence to study photosynthetic reactions as a function of the aforementioned variables. This research implies that chlorophyll <i>a</i> fluorescence data can be used to determine precise light conditions that help photoautotrophic organisms optimally function.