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<strong>Introduction:</strong> This study was conducted to evaluate the influence of chitosan Nano-gel/emulsion coating functionalized by<em> Ziziphora clinopodioides </em>essential oil (ZCEO) and nisin on growth inhibition of <em>Escherichia coli </em>O₁₅₇: H₇ inoculated in beef samples during 16 days in cold storage condition (4˚C). <strong>Methods: </strong>Beef sample was divided into six groups after inoculation of <em>E. coli </em>O₁₅₇:H₇. Treatments including control (no coating), chitosan 2%, sonicated chitosan 2%, Nano-emulsion of chitosan coating containing ZCEO (0.5%), Nano-gel of chitosan coating containing nisin (200 IU/g), Nano-emulsion of chitosan coating containingZCEO (0.5%) and nisin (200 IU/g) were stored at refrigeration temperature and bacterial count were performed on days: 0, 1, 2, 4, 8, 12 and 16. Data were analyzed using repeated measure ANOVA and Bonferroni post hoc tests. <strong>Results: </strong>Result indicated a significant reduction in <em>E. coli </em>O₁₅₇:H₇count in all treatments when compared to control group and the highest inhibitory activity was observed in chitosan Nano-emulsion coating containing ZCEO (0.5%) and nisin (200 IU/g). <strong>Conclusion:</strong> Accordingly, it is suggested that chitosan Nano-emulsion coating with ZCEO and nisinpractically be applied in beef to increase its safety against pathogenic bacteria especially <em>E. coli </em>O₁₅₇: H₇.