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Thyroid cancer is the most abundant tumor of the endocrine organs. Poorly differentiated thyroid cancer is still difficult to treat. Human cells exposed to long-term real (r-) and simulated (s-) microgravity (&#181;<i>g</i>) revealed morphological alterations and changes in the expression profile of genes involved in several biological processes. The objective of this study was to examine the effects of short-term &#181;<i>g</i> on poorly differentiated follicular thyroid cancer cells (FTC-133 cell line) resulting from 6 min of exposure to &#181;<i>g</i> on a sounding rocket flight. As sounding rocket flights consist of several flight phases with different acceleration forces, rigorous control experiments are mandatory. Hypergravity (hyper-<i>g</i>) experiments were performed at 18<i>g</i> on a centrifuge in simulation of the rocket launch and s-&#181;<i>g</i> was simulated by a random positioning machine (RPM). qPCR analyses of selected genes revealed no remarkable expression changes in controls as well as in hyper-<i>g</i> samples taken at the end of the first minute of launch. Using a centrifuge initiating 18<i>g</i> for 1 min, however, presented moderate gene expression changes, which were significant for <i>COL1A1</i>, <i>VCL</i>, <i>CFL1</i>, <i>PTK2</i>, <i>IL6,</i> <i>CXCL8</i> and <i>MMP14</i>. We also identified a network of mutual interactions of the investigated genes and proteins by employing <i>in-silico</i> analyses. Lastly, &#181;<i>g</i>-samples indicated that microgravity is a stronger regulator of gene expression than hyper-<i>g</i>.