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To review the microbiological profile of ulcerative keratitis associated with contact lens (CL)-wear, 35 patients with culture-proven CL-associated microbial keratitis were studied between September 1999 and September 2002. Corneal scrapes and CL-care products were collected and were subjected to microbiological evaluation. Gram-negative bacilli alone were recovered from the corneal scrapes of all 35 (100&#x0025;) patients, all 70 (100&#x0025;) CL storage case wells and also from CL-care solution of six (17.14&#x0025;) of the 35 patients. There was a significantly higher number of <i> Pseudomonas aeruginosa</i> (71.43&#x0025;) isolated from eyes with CL-related keratitis than other bacterial isolates (28.57&#x0025;) (<i> P</i> &#60; 0.001). Microbial contamination of CL storage cases was a great risk for gram-negative bacterial infection among soft CL-wearers.