Performance Evaluation of Different RT-PCR Kits for the Direct Detection of SARS-CoV-2 in Preheated Specimens
oleh: Rajeev Kumar Jain, Nagaraj Perumal, Deepti Chaurasia, Rakesh Shrivastava, Kamlesh Kumar Ahirwar, Archa Sharma, Garima Kapoor, Jaya Lalwani
| Format: | Article |
|---|---|
| Diterbitkan: | Thieme Medical and Scientific Publishers Pvt. Ltd. 2023-09-01 |
Deskripsi
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has created high demand for molecular kits and consumables for mass screening of suspected individuals. Direct real-time polymerase chain reaction (RT-PCR) assay without nucleic acid extraction has several advantages in saving testing time and cost and helps in the rapid reporting of SARS-CoV-2. The present study evaluated the analytical performance of four SARS-CoV-2 RT-PCR for direct RT-PCR testing using preheated specimens.