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The imaging spectroscopy mission EnMAP aims to assess the state and evolution of terrestrial and aquatic ecosystems, examine the multifaceted impacts of human activities, and support a sustainable use of natural resources. Once in operation (scheduled to launch in 2019), EnMAP will provide high-quality observations in the visible to near-infrared and shortwave-infrared spectral range. The scientific preparation of the mission comprises an extensive science program. This special issue presents a collection of research articles, demonstrating the potential of EnMAP for various applications along with overview articles on the mission and software tools developed within its scientific preparation.