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The aim of this study was to assess the expression of Th17-associated gene, AMPs genes and SIRT-1 protein in patients with inflammatory bowel disease (IBD). Material and Methods: We studied a group of 20 IBD patients, together with 20 subjects served as controls. Colonoscopy, terminal ileoscopy, and colonoscopic biopsy were performed for histopathology diagnosis, and quantitative gene expression of Th17-associated gene, CAMP, Elafin and SLPI by real-time PCR. SIRT-1 protein level expression was assessed by western blot. Results: The expression of the four studied genes—elafin, SLPI, CAMP and Th17-associated gene—by relative quantification was higher in the patient group than in the control group. A statistically significant positive correlation was found between SLPI and elafin in the patient group (r= 0.8325 P<0.001). A statistically significant positive correlation was reported as well between CAMP levels and elafin levels in the patient group (r=0.6842, P<0.001). In addition, CAMP levels had a positive correlation with SLPI levels in the patient group (r=0.6373, P<0.001). The highest expression of SIRT-1 was found in severe cases of IBD and the lowest expression was demonstrated in control subjects. A statistically significant positive correlation was detected between IL-17 levels and SIRT-1 levels in the patient group (r=0.7822, P<0.001). Conclusion: A high expression of Th17-associated gene and AMPs gene has a significant impact on clinical assessment of patients with IBD. SIRT may participate in the progression of IBD.