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Genus Glaucium belongs to papaveraceae. We studied two species of Glaucium. Glaucium leiocarpum Boiss. is synonym of Glaucium oxylobum Boiss. & Buhse in Flora Iranica while it is seprated in Flora orientalis, Flora U.S.S.R and Flore DeL’iran. Current  research is large studies in morphological study in vegetative and reproductive organs in two species. For further assurance, comparative anatomic in several organs and micromorphologic technical in pollen and seed and their differences is done. Samples were fresh or herbarium spacimen.Herbarium specimen from Herbarium of Plant Protection Research Institute of Iran and Islamic Azad University, North Tehran Branch were prepared. Our results indicated that, two species are differented together in morphology, comparative anatomic in several organs and micromorphological study in pollen and seed. In conclusion, two species are distinct, base on other floras and this result.