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Purpose The aim of the study was to evaluate the accuracy of intraocular lens (IOL) power calculation using partial coherence interferometry and OKULIX ray tracing software in high myopic cataract patients. Patients and methods This retrospective study was conducted on 60 consecutive myopic eyes with an axial length of at least 26.5 mm (60 patients, 20 men and 40 women, mean age 62.27±7.74 years). The patients underwent uneventful phacoemulsification and IOL implantation following IOL power calculation using the OA-1000 optical biometer and OKULIX ray tracing software for lens power calculation. All patients were recruited from the Department of Ophthalmology, Faculty of Medicine, Alexandria University (Alexandria, Egypt). All surgeries were performed by the same surgeon (K.M.N.). The accuracy of IOL power calculation was evaluated by subtracting attempted and achieved spherical refraction 1 month postoperatively and was recorded as mean absolute error. Results Fifty-three (88.33%) eyes yielded a postoperative spherical refraction that differed 1.00 D or less from predicted. In 42 (70%) eyes, the postoperative refractive error was within ±0.50 D. The mean absolute error was 0.45±0.40 D. Conclusion Optical biometry combined with OKULIX ray tracing software for calculation of IOL power provides accurate results for highly myopic cataract patients.