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The objective of this work was to obtain and characterize sodium alginate-based biopolymer films with the addition of cashew nut shell liquid (CNSL). The study employed a completely randomized design, including 0%, 0.5%, 1%, and 1.5% inclusion of CNSL. Uniform formation of the films was observed, and the addition of CNSL provided better thermal resistance than did the treatment without inclusion, while the addition of CNSL reduced the homogeneity of the microstructure, especially for the 1.5% inclusion level. The permeability of the film increased as the level of CNSL increased, especially in response to the concentrations of 1% and 1.5%, and no significant difference in permeability was observed between these treatments. The tensile strength decreased proportionally as a function of the addition of CNSL, as its inclusion increased the elasticity and elongation of the films. In addition, the films with CNSL demonstrated strong antioxidant activity and discrete antimicrobial activity, and ecotoxicity analysis showed that the levels of CNSL tested and the films produced were nontoxic. Thus, these films are promising and self-sustainable alternatives for the agrifood industry.