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Rapid Oxford Nanopore Technologies MinION Sequencing Workflow for <i>Campylobacter</i> <i>jejuni</i> Identification in Broilers on Site—A Proof-of-Concept Study
oleh: Clara Marin, Francisco Marco-Jiménez, Llucia Martínez-Priego, Griselda De Marco-Romero, Vicente Soriano-Chirona, Laura Lorenzo-Rebenaque, Giuseppe D’Auria
Format: | Article |
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Diterbitkan: | MDPI AG 2022-08-01 |
Deskripsi
<i>Campylobacter</i> is recognised as one of the most important foodborne bacteria, with a worldwide health and socioeconomic impact. This bacterium is one of the most important zoonotic players in poultry, where efficient and fast detection methods are required. Current official culture methods for <i>Campylobacter</i> enumeration in poultry usually include >44 h of culture and >72 h for identification, thus requiring at least five working shifts (ISO/TS 10272-2:2017). Here, we have assembled a portable sequencing kit composed of the Bento Lab and the MinION and developed a workflow for on-site farm use that is able to detect and report the presence of <i>Campylobacter</i> from caecal samples in less than five hours from sampling time, as well as the relationship of <i>Campylobacter</i> with other caecal microbes. Beyond that, our workflow may offer a cost-effective and practical method of microbiologically monitoring poultry at the farm. These results would demonstrate the possibility of carrying out rapid on-site screening to monitor the health status of the poultry farm/flock during the production chain.