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Polymorphism and epitope sharing between the alleles of merozoite surface protein-1 of <it>Plasmodium falciparum </it>among Indian isolates
oleh: Tyagi Prajesh K, Sharma Surya K, Diwan Suraksha S, Sunil Sujatha, Mamillapalli Anitha, Adak Tridibes, Joshi Hema, Malhotra Pawan
Format: | Article |
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Diterbitkan: | BMC 2007-07-01 |
Deskripsi
<p>Abstract</p> <p>Background</p> <p>The C-terminal region of merozoite surface protein-1 (MSP-1) is one of the leading candidates for vaccination against the erythrocytic stages of malaria. However, a major concern in the development of MSP-1 based malaria vaccine is the polymorphism observed in different geographical <it>Plasmodium falciparum </it>isolates. To explore whether the sequence heterogeneity of PfMSP-1 leads to variation in naturally acquired anti-MSP-1<sub>19 </sub>antibodies, the present study was undertaken to study PfMSP-1<sub>19 </sub>sequence polymorphism in malaria-endemic villages in eastern India and also carried out a competition enzyme-linked immunosorbent assay using three PfMSP-1<sub>19 </sub>variant forms.</p> <p>Methods</p> <p>The sequence variations in the C-terminal region of PfMSP-1<sub>19 </sub>were determined in a malaria endemic region. Three PfMSP-1<sub>19 </sub>variants were produced in <it>Escherichia coli </it>(PfMSP1<sub>19</sub>QKNG-L, PfMSP1<sub>19</sub>EKNG-L and PfMSP1<sub>19</sub>ETSR-F) and an immunodepletion assay was carried out using the corresponding patients' sera.</p> <p>Results</p> <p>Results revealed predominance of PfMAD20 allele among Indian field isolates. Seven PfMSP-1<sub>19 </sub>variant forms were isolated in a singe geographical location. Three of PfMSP-1<sub>19 </sub>variant forms when expressed in <it>E. coli </it>showed presence of cross-reaction as well as variant specific antibodies in malaria infected patient sera.</p> <p>Conclusion</p> <p>The present study demonstrates the existence of allele specific antibodies in <it>P. falciparum</it>-infected patient sera, however their role in protection requires further investigation. These results thereby, suggest the importance of a multi-allelic PfMSP-1<sub>19 </sub>based vaccine for an effective malaria control.</p>