Performance of direct immunofluorescence assay for the detection of human metapneumovirus under clinical laboratory settings
oleh: Jonas Michel Wolf, Tatiana Schäffer Gregianini, Claudete Maria Farina Seadi, Gabriela Luchiari Tumioto, Bibiana Paula Dambrós, Fernanda Kieling Moreira Lehmann, Silvia De Carli, Nilo Ikuta, Vagner Ricardo Lunge
| Format: | Article |
|---|---|
| Diterbitkan: | Sociedade Brasileira de Medicina Tropical (SBMT) 2015-12-01 |
Deskripsi
ABSTRACT INTRODUCTION: Human metapneumovirus (hMPV) is an emergent human respiratory pathogen. This study aimed to evaluate the performance of direct immunofluorescence (DIF) to detect hMPV in a clinical laboratory setting. METHODS: Nasopharyngeal aspirate samples (448) of children and adults with respiratory illness were used to detect hMPV by using DIF and real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. RESULTS: In all, 36 (8%) samples were positive by DIF and 94 (21%) were positive by qRT-PCR. Direct immunofluorescence specificity was 99% and sensitivity was 38%. CONCLUSIONS: DIF is not very sensitive under clinical laboratory settings.