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Pineapple (<i>Ananas comosus</i> L.) is an important fruit crop in tropical regions, and it requires efficient sugar allocation during fruit development. Sugars Will Eventually be Exported Transporters (SWEETs) are a group of novel sugar transporters which play critical roles in seed and fruit development. However, the function of <i>AcSWEETs</i> remains unknown in the sugar accumulation. Herein, 17 <i>AcSWEETs</i> were isolated and unevenly located in 11 chromosomes. Analysis of a phylogenetic tree indicated that 17 genes were classified into four clades, and the majority of <i>AcSWEETs</i> in each clade shared similar conserved motifs and gene structures. Tissue-specific gene expression showed that expression profiles of <i>AcSWEETs</i> displayed differences in different tissues and five <i>AcSWEETs</i> were strongly expressed during fruit development. <i>AcSWEET11</i> was highly expressed in the stage of mature fruits in ‘Tainong16’ and ‘Comte de paris’, which indicates that <i>AcSWEET11</i> was important to fruit development. Subcellular localization analysis showed that AcSWEET11 was located in the cell membrane. Notably, overexpression of <i>AcSWEET11</i> could improve sugar accumulation in pineapple callus and transgenic tomato, which suggests that <i>AcSWEET11</i> might positively contribute to sugar accumulation in pineapple fruit development. These results may provide insights to enhance sugar accumulation in fruit, thus improving pineapple quality in the future.