Atypical carcinoid and large cell neuroendocrine carcinoma of the lung: a proteomic dataset from formalin-fixed archival samples
oleh: Alessandro Tanca, Maria Filippa Addis, Salvatore Pisanu, Marcello Abbondio, Daniela Pagnozzi, Albino Eccher, Guido Rindi, Paolo Cossu-Rocca, Sergio Uzzau, Giuseppe Fanciulli
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2016-06-01 |
Deskripsi
Here we present a dataset generated using formalin-fixed paraffin-embedded archival samples from two rare lung neuroendocrine tumor subtypes (namely, two atypical carcinoids, ACs, and two large-cell neuroendocrine carcinomas, LCNECs). Samples were subjected to a shotgun proteomics pipeline, comprising full-length protein extraction, SDS removal through spin columns, in solution trypsin digestion, long gradient liquid chromatography peptide separation and LTQ-Orbitrap mass spectrometry analysis. A total of 1260 and 2436 proteins were identified in the AC and LCNEC samples, respectively, with FDR <1%. MS data are available in the PeptideAtlas repository at http://www.peptideatlas.org/PASS/PASS00375. Keywords: Neuroendocrine tumors, Proteomics, FFPE, Archival tissues, Mass spectrometry