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Nanoplastics (NPs) are widely concerned because of their potential adverse effects on marine organisms. However, the detection and quantification of NPs are challenging due to their small size and weak signals. In this study, the ac-cumulation of Pd-doped polystyrene (PS) NPs in the digestive tract of Sebastes schlegelii was investigated by an inductively coupled plasma mass spectrometer (ICP-MS). Results showed that NPs could be accumulated in the digestive tract and faeces after 3-day exposure with concentrations of 0.5, 5, and 10 mg/L. The highest accumulation of NPs was in the digestive tract, up to 0.23 ± 0.02 mg/g wet wt after 2 days. However, the highest accumulation of NPs was found in faeces (29.59 ± 0.54 mg/g wet wt) after 3-day exposure. Moreover, the digestive tract and faeces showed a concentration-dependent of NPs, which may lead to the transfer of NPs to higher trophic levels. The present study provides evidence for the bioaccumulation of NPs in organisms and the potential impact of NPs on ecological risk.