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Comparative intradermal test was done in an organised dairy farm to detect Johnin and Tuberculin positive reactors. Hematological and biochemical parameters of the reactors were compared with healthy animals. Haematological studies of Johnin and Tuberculin reactors of bovines revealed reduction in the values of packed cell volume, total erythrocyte count and an increase in the erythrocyte sedimentation rate and total leucocyte count. Biochemical parameters showed a decrease in the serum iron content of tuberculin reactors. Comparative clinical pathology related to hematological and biochemical, parameters of both reactors are described