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Inhibition of <i>Salmonella</i> by <i>Lactobacillus</i> has been a popular research topic for decades; however, the inhibition potential of chicken-derived <i>Salmonella</i> by chicken-derived <i>Lactobacillus</i> has not yet been studied. In this study, 89 strains of <i>Lactobacillus</i> from chicken intestines were isolated by national standard method, Gram staining, physiological, and biochemical experiments and molecular sequencing; The inhibition characteristics of 89 strains of chicken derived <i>Lactobacillus</i> against 10 strains <i>Salmonella</i> (<i>S</i>. Enteritidis SE05, SC31, SC21, SC72 SC74, SC79, SC83, SC87; <i>S</i>. bongori SE47; <i>S</i>. Typhimurium, SC85) were detected by agar inhibition zone, The results showed that the inhibition zone of 24 strains of chicken derived Lactobacillus was more than 10 mm, which indicated that the isolated chicken derived <i>Lactobacillus</i> could effectively inhibit the growth of <i>Salmonella</i>; The drug resistance and bile salt tolerance of these 24 strains were analyzed, The results showed that the standard strains LG and L76 were not resistant, and the other 22 <i>Lactobacillus</i> strains showed different degrees of resistance. The strains LAB24, LAB26, LAB53, LAB69, and L76 showed good tolerance at the concentration of 3 g/L bile salt; Caco-2 cell experiment and flow cytometry were used to analyze the inhibitory effect of chicken derived <i>Lactobacillus</i> on the adhesion of <i>Salmonella</i> to Caco-2 cells, The results showed that 16 probiotics could effectively inhibit the adhesion of <i>Salmonella</i> to Caco-2 cells. Twelve probiotics were identified by molecular biology. The results showed that L76 was <i>Enterococcus faecalis</i>, and the other 11 strains were <i>Lactobacillus.</i>