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This study aimed to explore the safety and properties of <i>Lacticaseibacillus paracasei</i> IDCC 3401 as a novel probiotic strain via genomic and phenotypic analyses. In whole-genome sequencing, the genes associated with antibiotic resistance and virulence were not detected in this strain. The minimum inhibitory concentration test revealed that <i>L. paracasei</i> IDCC 3401 was susceptible to all the antibiotics tested, except for kanamycin. Furthermore, the strain did not produce toxigenic compounds, such as biogenic amines and D-lactate, nor did it exhibit significant toxicity in a single-dose acute oral toxicity test in rats. Phenotypic characterization of carbohydrate utilization and enzymatic activities indicated that <i>L. paracasei</i> IDCC 3401 can utilize various nutrients, allowing it to grow in deficient conditions and produce health-promoting metabolites. The presence of <i>L. paracasei</i> IDCC 3401 supernatants significantly inhibited the growth of enteric pathogens (<i>p</i> < 0.05). In addition, the adhesion ability of <i>L. paracasei</i> IDCC 3401 to intestinal epithelial cells was found to be as superior as that of <i>Lacticaseibacillus rhamnosus</i> GG. These results suggest that <i>L. paracasei</i> IDCC 3401 is safe for consumption and provides health benefits to the host.