Extra kinetic dimensions for label discrimination
oleh: Raja Chouket, Agnès Pellissier-Tanon, Aliénor Lahlou, Ruikang Zhang, Diana Kim, Marie-Aude Plamont, Mingshu Zhang, Xi Zhang, Pingyong Xu, Nicolas Desprat, Dominique Bourgeois, Agathe Espagne, Annie Lemarchand, Thomas Le Saux, Ludovic Jullien
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2022-03-01 |
Deskripsi
Label discrimination is challenging in fluorescence microscopy due to broad spectra and narrow lifetime distribution. Here, the authors introduce extra kinetic dimensions by illuminating reversibly photoswitchable fluorophores with different intensities, and discriminate 20 spectrally similar fluorophores.