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In this research work, we attempted to know the positive response of putrescine on plant growth and flowering of annual flower gypsophila. Putrescine is important because it has been shown that exogenous treatment can enhance the production or accumulation of bioactive chemicals that show a positive impact on plant growth and development. Significant results were obtained in various growth and flowering parameters of annual gypsophila due to foliar treatment with putrescine. Maximum plant height, maximum plant spread, maximum number of leaves per plant, maximum number of primary branches and secondary branches per plant at 60 DAT and 90 DAT as well as maximum number of flower clusters per branch and maximum number of flower clusters per plant was recorded from 1.50 mmol of putrescine application.