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Actinomyces bacteria are the best parser microrganisms in environment and agriculture. These bacteria are frequent in compost and produce antibiotics & extracellular enzymes. In this study, some strains of actinomycetes were separated from different steps in composting process. After that, morphological, biochemical and molecular tests were performed for identification of them. Carboxy Methyl Cellulase assay was applied for endoglucanase activity. For optimizing conditions different treatments including<em> </em>pHs: 5.7, 6, 6.5, 7, 7/5, temperatures 45-65°C and times 30, 60 and 90 minutes were used. Optimum temperature for high endoglucanase activity was 60°C and isolate No.19 with 0.289 unit/ml showed the highest endoglucanase activity in this temperature. According to molecular result obtained from RFLP of 16SrRNA, most of actinomyces isolates were from<em> streptomyces </em>genus.