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The eukaryotic initiation factor 4E binding protein (4E-BP) family is involved in translational control of cell proliferation and pro-angiogenic factors. The zebrafish eukaryotic initiation factor 4E binding protein 3 like (<i>eif4ebp3l</i>) is a member of the 4E-BPs and responsible for activity-dependent myofibrillogenesis, but whether it affects cardiomyocyte (CM) proliferation or heart regeneration is unclear. We examined <i>eif4ebp3l</i> during zebrafish vascular development and heart regeneration post cryoinjury in adult zebrafish. Using morpholino injections we induced silencing of <i>eif4ebp3l</i> in zebrafish embryos, which led to increased angiogenesis at 94 h post fertilization (hpf). For investigation of <i>eif4ebp3l</i> in cardiac regeneration, zebrafish hearts were subjected to cryoinjury. Regenerating hearts were analyzed at different time points post-cryoinjury for expression of <i>eif4ebp3l</i> by in situ hybridization and showed strongly decreased <i>eif4ebp3l</i> expression in the injured area. We established a transgenic zebrafish strain, which overexpressed <i>eif4ebp3l</i> under the control of a heat-shock dependent promotor. Overexpression of <i>eif4ebp3l</i> during zebrafish heart regeneration caused only macroscopically a reduced amount of fibrin at the site of injury. Overall, these findings demonstrate that silencing of <i>eif4ebp3l</i> has pro-angiogenic properties in zebrafish vascular development and when <i>eif4ebp3l</i> is overexpressed, fibrin deposition tends to be altered in zebrafish cardiac regeneration after cryoinjury.