Generation of an induced pluripotent stem cell line (CSBZZUi001-A) from a female Alzheimer’s patient carrying the PSEN1 709 T > C heterozygous mutation
oleh: Xiao-Yan Meng, Hui-Fang Sun, Ying-Jie Zhang, Wen-Xiu Li, Dian-Dian Wang, Dan-Hua Liu, Sen-Miao Li, Yue Wu, Jia-Kang Li, Xiao-Hui Liu, Pei-Pei Liu, Run-Zhou Yang, Shu-Ang Li, Jian-Sheng Kang
| Format: | Article |
|---|---|
| Diterbitkan: | Elsevier 2024-09-01 |
Deskripsi
Pluripotent stem cells were generated through the electroporation of episomal plasmids, containing crucial reprogramming factors, into skin fibroblasts extracted from a female Alzheimer’s patient harboring the PSEN1 709 T > C (p.Phe237Leu) heterozygous mutation. The pluripotent stem cells exhibit a normal karyotype and express pivotal stem cell markers including TRA-1-60, Nanog, SOX2, and OCT4. Furthermore, their capacity to differentiate into the three germ layers in in vivo teratoma experiments has been substantiated. The pluripotent stem cell line can serve as a cellular model for Alzheimer’s disease, offering significant value in elucidating the pathogenesis and therapeutic strategies of the disease.