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Changes in the colostrum nutritive value were studied in dairy cows of the Czech Red Pied x Holstein (n = 10) from the 2nd to 72nd hour post partum. Of them, five and five animals were in the first and the second (or higher) lactation, respectively. Dry matter (DM) content ranged from 20.37 to 13.78% (mean value 15.61%). Within the first 12 and 72 hours after parturition, the shares of crude protein in DM were more than 60% (14.33 – 9.71%) and nearly 50% (6.34 – 4.06%; mean 5.61%), respectively. Milk fat made one quarter (2.29 - 5.16%; mean 3.72%) and lactose approximately 22% (2.74 - 3.93%; mean 3.43%) of DM content, respectively.