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In 2006 and 2007 samples of harvested grain were used to verify the possibility of distinguishing 12 winter wheat genotypes and of detecting impurities on the basis of polymorphism of prolamin kernel proteins. Uniform electrophoretic patterns were detected in genotypes of Athlet, Buteo, Dromos, Ebi, Etela, Florett, Livia, Simila wheat in 2006 and 2007. On the basis of the identity index two sister prolamin lines with different share, depending on the year of harvest, were discovered in 3 wheat genotypes (Astella, Brea and Hana). The proportion of sister gliadin lines in the Astella and Brea ge­no­ty­pes was the same in both years. There was only minor difference (± 2.5%) in the share of the sister lines in the Hana variety between the respective years, and could be influenced by environmental factors. A foreign genotype was detected in the Mona variety. The identity index of the impurity to the Mona variety (ii = 0.30) was considerably low. In the impurity the gliadin block Gld 1B3 was not detected, which is the genetic marker of rye translocation T1BL.1RS (the Sr31 gene of resistance to black rust, higher cold resistance and the marker of poor baking quality – presence of secalin genes). The results proved the potential practical application of the electrophoretic detection of polymorphism of prolamin proteins as markers of impurities of foreign genotypes in a seed sample.