Identification of an Integrase That Responsible for Precise Integration and Excision of Riemerella anatipestifer Genomic Island
oleh: Ying Wang, Yang Zhang, Yijie Cui, Zhijian Sun, Zutao Zhou, Zutao Zhou, Zutao Zhou, Sishun Hu, Sishun Hu, Sishun Hu, Shaowen Li, Shaowen Li, Shaowen Li, Mei Liu, Mei Liu, Mei Liu, Xianrong Meng, Xianrong Meng, Xianrong Meng, Yuncai Xiao, Yuncai Xiao, Yuncai Xiao, Deshi Shi, Deshi Shi, Deshi Shi, Dingren Bi, Dingren Bi, Dingren Bi, Zili Li, Zili Li, Zili Li
| Format: | Article |
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| Diterbitkan: | Frontiers Media S.A. 2019-09-01 |
Deskripsi
Riemerella anatipestifer is a Gram-negative, pathogenic bacterium, which is harmful to poultry. However, the genomic islands (GIs) in R. anatipestifer are not well-studied. In this study, a 10K genomic island was predicted by the bioinformatics analysis of R. anatipestifer ATCC 11845, which is widely found in other R. anatipestifer genomes. We had first reported the genomic island integration and excision function in R. anatipestifer. We successfully constructed the integration plasmid by using the integrase and 53 bp attP elements. The 10K GI was found integrated at the 53 bp attB located in the Arg-tRNA of the R. anatipestifer RA-YM chromosome. We identified an integrase that helped in the precise integration and excision in R. anatipestifer and elucidated the molecular mechanism of the 10K genomic island integration and excision. Furthermore, we provided a new method for the gene expression and construction of complementary strain.