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Objective: To standardize the technique of induced breeding and spawning of green mussel Perna viridis (P. viridis), in captivity. Methods: In Experiment-A, the temperature was increased at a rate of 2 °C/5 days interval. In Experiment-B, a rise of 3 °C/5 days was practiced, whereas in Experiment C and D, respectively 4 and 5 °C was increased in 5 days interval. The temperature was maintained constant at 20 °C in the Control. Results: The increase in temperature showed a progressive effect on the gonadal development of mussels. The gonads ripped at 30 to 32 °C in all the experimental tanks, irrespective of the difference in temperature hike. Complete spawning in P. viridis was achieved by gradually raising the temperature from 20 to 35 °C at a rate of 3 or 4 °C/5 days. Conclusion: According to the present study temperature induced spawning method is very simple and cost effective and can accelerate the production of mussel seeds in hatchery units and further stock improvement through genetic manipulation.