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Urban beekeeping has gained salience because of its significance in biodiversity conservation and community building. Despite this, beekeeping practices in urban areas have received negative perceptions from residents, which stem from public safety concerns. There is, therefore, a need to enhance and/or work on appropriate rules for maximizing the profits while minimizing the risks. Amongst the present regulations, the installation of barriers and setbacks is the most common rule for public safety. However, only a limited number of empirical studies have reported on their effective location and height. Thus, in this study, an experimental apiary was set up with different types of barriers installed with varying distances to observe and measure flyway patterns of honey bees. We used a 3D laser scanner, which obtained 8529 points of highly accurate flight location data in about five hours. Results showed that the heights (1.8 and 0.9 m) of the barriers installed were effective in increasing the flight altitudes. The distance of the fence, which was installed as close as 1 m from the hives, was effective as well. These findings, which showed that barriers and setbacks are effective, can have regulatory implications in designing apiaries in urban spaces, where location is often restricted.