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ATM: To investigate the relationship between the oculus dominans and the eye with higher diopter among myopes, and to study the difference between the Worth 4 dot test and hole-in-the-card test. <p>METHODS: Totally 125 cases were divided into two groups as physiogenic anisometropic group and pathological anisomitropic group according to the degree of binoculus anisometropy. Worth 4 dot test and hole-in-the-card test were used to distinguish oculus dominans respectively based on medical optometry.<p>RESULTS: In physiogenic anisometropic group, the oculus dominans and the eye with higher diopter among myopes had significant relationship by hole-in-the-card test(<i>Z</i> = -4.057, <i>P</i><0.01)and Worth 4 dot test(<i>Z</i>=-3.558, <i>P</i><0.01). However, in pathological group, No statistical significance was between two tests(<i>P</i>>0.05); distinguish the oculus dominans had no statistically significant difference between the Worth 4 dot test and hole-in-the-card test(<i>n</i>=125, <i>P</i>>0.05). <p>CONCLUSION:The oculus dominans is determined by gene or formed during juvenile period, then myopia anisometropic may affect the choice of that. The Worth 4 dot test has some reference significance for observing oculus dominans.