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Nylanderia flavipes (Smith, 1874) is a Formicine ant found in East Asia. We have completed mitochondrial genome of N. flavipes of which length is 16,687 bp including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, and a control region. The base composition was AT-biased (GC ratio is 18.9%). Gene order of N. flavipes presents a unique inversion of trnP in comparison to Camponotus species. Our phylogenetic trees together with phylogenomic analysis suggest that the gene order rearrangements occurred independently in Camponotus and N. flavipes.