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Fusarium wilts of linseed caused by Fusarium oxysporum f.sp. lini have been identified in nearly all linseed-producing countries of the world. A comparison of phytoextract, chemical, and bio control agents against Fusarium oxysporum f.sp. lini was conducted. Among the phytoextracts tested, Neem extract exhibited the highest antifungal activity in inhibiting the growth of F. oxysporum f.sp. lini at 5, 15, and 30% concentrations. In terms of biocontrol agents, T. virens was identified as the most efficient antagonist against F. oxysporum f.sp. lini. It significantly inhibited pathogen mycelial growth, displaying the highest level of inhibition. Among the chemical fungicides assessed, propiconazole exhibited the lowest mycelial growth of the pathogen and outperformed the other fungicides, with difenoconazole following as the next most effective.