Protein identification by 3D OrbiSIMS to facilitate in situ imaging and depth profiling
oleh: Anna M. Kotowska, Gustavo F. Trindade, Paula M. Mendes, Philip M. Williams, Jonathan W. Aylott, Alexander G. Shard, Morgan R. Alexander, David J. Scurr
| Format: | Article |
|---|---|
| Diterbitkan: | Nature Portfolio 2020-11-01 |
Deskripsi
Label-free protein characterization at surfaces requires digestion or matrix application prior to mass spectrometry. Here, the authors report the assignment of undigested proteins at surfaces by de novo sequencing and apply the methodology to a protein monolayer biochip and for in situ depth profiling of proteins through human skin.